Difference between revisions of "Part:BBa K108031:Design"
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===Source=== | ===Source=== | ||
| − | ... | + | CheZ was cloned from E.coli K12 DH10B genome(reference 4). |
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| + | T7 terminator was cloned from pET-15b(Novagen). | ||
===References=== | ===References=== | ||
Revision as of 15:27, 24 October 2008
TARS-CheZ
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 31
Design Notes
LacI Operator sequence was designed according to reference 1.
TARS sequence was designed according to reference 2.
LVA sequence was designed according to reference 3.
PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.
Source
CheZ was cloned from E.coli K12 DH10B genome(reference 4).
T7 terminator was cloned from pET-15b(Novagen).