Difference between revisions of "Part:BBa K3254024"

 
 
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<partinfo>BBa_K3254024 short</partinfo>
 
<partinfo>BBa_K3254024 short</partinfo>
  
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This is a superfold-GFP translational unit. The RiboJ can insulate the interference by different 5'-UTR sequence. As a result, the sfGFP expression amount can be used for calibrating the transcriptional activity of its upstream sequence without considering the different translational rate caused by the upstream RNA sequence.
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This is a superfold-GFP translational unit. The [[Part:BBa_K2615996|RiboJ]] can insulate the interference by different 5'-UTR sequence. As a result, the sfGFP expression amount can be used for calibrating the transcriptional activity of its upstream sequence without considering the different translational rate caused by the upstream RNA sequence.
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=Usage=
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*This part can be used for quantitative testing the characteristics of transcriptional regulation device.
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*The [[Part:BBa_K3254025|Ptac-sfGFP]] is a good example for such testing.
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*You can see the page of [[Part:BBa_K3254025|BBa_K3254025]] for getting more information.
  
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===Usage and Biology===
 
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3254024 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3254024 SequenceAndFeatures</partinfo>

Latest revision as of 02:58, 21 October 2019


RiboJ-B0034-sfgfp

This is a superfold-GFP translational unit. The RiboJ can insulate the interference by different 5'-UTR sequence. As a result, the sfGFP expression amount can be used for calibrating the transcriptional activity of its upstream sequence without considering the different translational rate caused by the upstream RNA sequence.

Usage

  • This part can be used for quantitative testing the characteristics of transcriptional regulation device.
  • The Ptac-sfGFP is a good example for such testing.
  • You can see the page of BBa_K3254025 for getting more information.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 114