Difference between revisions of "Part:BBa K2940002"

 
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<partinfo>BBa_K2940002 short</partinfo>
 
<partinfo>BBa_K2940002 short</partinfo>
  
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Improved Bpul  
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Improved Bpul (bacterial laccase Bpul from Bacillus pumilus)
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
  
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Laccases are enzymes with the potential to biodegrade synthetic dyes with different chemical structures. These enzymes are able to oxidize a wide range of phenolic substrates without the presence of additional co-factors. This improved Bpul enzyme has a major bioactivity for degrading azo dyes.
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===Characterization===
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Libraries of laccase mutants were generated using error prone Taq PCR (New England BioLabs). The mutant libraries of assembled PelB and Bpul were then cloned into pET28a-GG-RFP-CD via Golden Gate cloning for expression.
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'''Characterization ABTS assay'''
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98 mutant laccase enzymes were assayed for enzymatic activity using ABTS assay. This assay showed four mutants with improved activity (one with a significant increase). This mutant was sequenced and showed eight-point mutations when compared to WT Bpul. 
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[[Image:T--Edinburgh OG--FigureScreening_Nathan.png|400px|]]
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'''Results'''
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[[Image:T--Edinburgh OG--FigureBpul2 Nathan.png|200px|]]
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2940002 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K2940003 SequenceAndFeatures</partinfo>
  
  
 
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<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K2940002 parameters</partinfo>
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<partinfo>BBa_K2940003 parameters</partinfo>
 
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Revision as of 02:30, 21 October 2019


Improved Bpul

Improved Bpul (bacterial laccase Bpul from Bacillus pumilus)

Usage and Biology

Laccases are enzymes with the potential to biodegrade synthetic dyes with different chemical structures. These enzymes are able to oxidize a wide range of phenolic substrates without the presence of additional co-factors. This improved Bpul enzyme has a major bioactivity for degrading azo dyes.


Characterization

Libraries of laccase mutants were generated using error prone Taq PCR (New England BioLabs). The mutant libraries of assembled PelB and Bpul were then cloned into pET28a-GG-RFP-CD via Golden Gate cloning for expression.

Characterization ABTS assay

98 mutant laccase enzymes were assayed for enzymatic activity using ABTS assay. This assay showed four mutants with improved activity (one with a significant increase). This mutant was sequenced and showed eight-point mutations when compared to WT Bpul.


T--Edinburgh OG--FigureScreening Nathan.png


Results


T--Edinburgh OG--FigureBpul2 Nathan.png

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 128
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 20
    Illegal SpeI site found at 128
    Illegal NotI site found at 27
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 128
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 128
  • 1000
    COMPATIBLE WITH RFC[1000]