Difference between revisions of "Part:BBa K2595000"

Revision as of 02:04, 21 October 2019

GcvB

GcvB is a small RNA composed by 205 nucleotides. This sRNA is expressed during early log phase, but its level decrease during cell growth. Its transcription is stimulated by GcvA and repressed by GcvR in response to glycine.

GcvB regulates the expression of specific transporters and proteins (eg. DppA, Oppa, CycA or transcription factor CsgD).The regulation mechanisms differ depending on the molecule being regulated: while both OppA and DppA are repressed at the level of translation initiation, for the regulation of CycA expression, GcvB needs to interact with Hfq. In Salmonella enterica, there are more than 20 GcvB targets, which justifies the major utility and functionality of this sRNA inside bacterial cells.

Mutant gcvB overproduces OppA and DppA, but maintains its functionality in the glycine cleavage system activity. The overexpression of GcvB increases biofilm formation and decreases swarming motility (Argaman et al., 2001).

Usage and Biology

This synthetic version of GcvB follows the same principles as its wild type partner to regulate gene expression. This modified construct, however, can target any gene of interest due to its sequence which contains a BaeI restriction enzyme site. After digestion with this restriction enzyme and ligation with a target sequence, GcvB can regulate the expression of the gene that contains the complementary regions for its targeting sequence. Ligation with the desired targeting sequence does not create any scar in the sRNA sequence.

Sequence and Features

Team KCL_UK 2019

“Team KCL_UK 2019 has improved this part and created a new part BBa_K2968000 (https://parts.igem.org/Part:BBa_K2968000)“