Difference between revisions of "Part:BBa K2960003"

This is a composite part consisting of a constitutively-active promoter sequence (J23100), a ribosome binding site (BBa_J61100), the mlrC gene (BBa_k2960008), the TorA tat sequence (BBa_K1012002), a 3x FLAG tag (BBa_K823034), and a terminator (BBa_B0015).  

This is a composite part consisting of a constitutively-active promoter sequence (J23100), a ribosome binding site (BBa_J61100), the mlrC gene (BBa_k2960008), the TorA tat sequence (BBa_K1012002), a 3x FLAG tag (BBa_K823034), and a terminator (BBa_B0015).  

A cassette of enzymes endogenous to Sphingopyxis sp. has been shown to sequentially break down microcystin-LR (Figure 1). The enzymes have been named mlrA, mlrB, mlrC, mlrD, mlrE, and mlrF. MlrC is the third enzyme in the microcystin-degradation pathway. MlrC is assumed to be a metallopeptidase. It cleaves the tetrapeptide intermediate into an Adda amino acid and other smaller peptides. The exact smaller peptides that result are still unknown.  

A cassette of enzymes endogenous to Sphingopyxis sp. has been shown to sequentially break down microcystin-LR (Figure 1). The enzymes have been named mlrA, mlrB, mlrC, mlrD, mlrE, and mlrF. MlrC is the third enzyme in the microcystin-degradation pathway. MlrC is assumed to be a metallopeptidase. It cleaves the tetrapeptide intermediate into an Adda amino acid and other smaller peptides. The exact smaller peptides that result are still unknown.  

Figure 1. Schematic of TorA twin-argenine translocation pathway altered to transport folded mlr proteins.

Figure 1. Schematic of TorA twin-argenine translocation pathway altered to transport folded mlr proteins.

<!-- -->

<!-- -->