Difference between revisions of "Part:BBa K847004"
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==RHIT 2019’s Characterisation== | ==RHIT 2019’s Characterisation== | ||
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Characterization of BBa_K847004, the protocol for which can be found in the lab notebook, shows that bacteria containing the SOD gene in this experiment have better survivability against the reactive oxygen species produced by copper, including a larger number of final CFUs, as well as survivability at higher concentrations of copper total. | Characterization of BBa_K847004, the protocol for which can be found in the lab notebook, shows that bacteria containing the SOD gene in this experiment have better survivability against the reactive oxygen species produced by copper, including a larger number of final CFUs, as well as survivability at higher concentrations of copper total. | ||
Revision as of 01:33, 21 October 2019
''Deinococcus radiodurans'' Superoxide dismutase, SodCu/Zn
Deinococcus radiodurans is an extremely radiation-resistant bacterium: while about 10 Gy (absorbed radiation dose, Gray) can kill most vertebrates, D. radiodurans can withstand up to 12,000 Gy. Current literature supports this finding with D. radiodurans’ unique genetic makeup which allows it to better handle radiation exposure. The two main effects of radiation exposure to bacterial cells are DNA damage and the creation of toxic superoxide species (Daly 2009). To take care of the pesky superoxides, superoxide dismutases are expressed in high levels in D. radiodurans (Slade and Miroslav 2011). These enzymes break the reactive species down into harmless oxygen and hydrogen peroxides. This dismutase uses copper and zinc as cofactors (Gao, Zhang, Song, Chen, and Zhong 2009).
RHIT 2019’s Characterisation
Characterization of BBa_K847004, the protocol for which can be found in the lab notebook, shows that bacteria containing the SOD gene in this experiment have better survivability against the reactive oxygen species produced by copper, including a larger number of final CFUs, as well as survivability at higher concentrations of copper total.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 389
Illegal NgoMIV site found at 496 - 1000COMPATIBLE WITH RFC[1000]