Difference between revisions of "Part:BBa K3320006"

Line 8: Line 8:
 
===Usage and Biology===
 
===Usage and Biology===
 
Our AND Gate has repressor-promoter relation, basically using two promoters to control the signal (repressor) production that represses our protein of interest.
 
Our AND Gate has repressor-promoter relation, basically using two promoters to control the signal (repressor) production that represses our protein of interest.
 
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 01:32, 21 October 2019


Multi-input AND-gate

This is a multi-input AND-gate for biosensor tumor microenvironment.

Usage and Biology

Our AND Gate has repressor-promoter relation, basically using two promoters to control the signal (repressor) production that represses our protein of interest.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 78
    Illegal NheI site found at 101
    Illegal NheI site found at 808
    Illegal NheI site found at 831
    Illegal NheI site found at 4308
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1415
    Illegal AgeI site found at 4057
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3284