Difference between revisions of "Part:BBa K3142012:Design"

 
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===Design Notes===
 
===Design Notes===
−
NO
+
The sequence has been optimized for lactic acid bacteria.
  
 +
===Source===
  
 +
===References===
 +
[1] Busby, S., and Ebright, R. H. (1999) Transcription Activation by Catabolite Activator 519
 +
Protein (CAP). J. Mol. Biol. 293, 199–213. 520
  
−
===Source===
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[2] Savery, N. J., Lloyd, G. S., Kainz, M., Gaal, T., Ross, W., Ebright, R. H., Gourse, R. L., and 521
 +
Busby, S. J. W. (1998) Transcription activation at Class II CRP-dependent promoters: identification of 522 determinants in the C-terminal domain of the RNA polymerase α subunit. EMBO J. 17, 3439–344
  
−
NO
+
[3] Lloyd, G. S., Busby, S. J. W., and Savery, N. J. (1998) Spacing Requirements for 529 interactions between the C-terminal domain of α subunit of Escherichia coli RNA polymerase and the 530
−
 
+
cAMP receptor protein. Biochem. J. 420, 413–420.
−
===References===
+

Latest revision as of 01:24, 21 October 2019


Glucose starvation promoter（PT-αcrp）


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 26
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 26
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 26
    Illegal BamHI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 26
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 26
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence has been optimized for lactic acid bacteria.

Source

References

[1] Busby, S., and Ebright, R. H. (1999) Transcription Activation by Catabolite Activator 519 Protein (CAP). J. Mol. Biol. 293, 199–213. 520

[2] Savery, N. J., Lloyd, G. S., Kainz, M., Gaal, T., Ross, W., Ebright, R. H., Gourse, R. L., and 521 Busby, S. J. W. (1998) Transcription activation at Class II CRP-dependent promoters: identification of 522 determinants in the C-terminal domain of the RNA polymerase α subunit. EMBO J. 17, 3439–344

[3] Lloyd, G. S., Busby, S. J. W., and Savery, N. J. (1998) Spacing Requirements for 529 interactions between the C-terminal domain of α subunit of Escherichia coli RNA polymerase and the 530 cAMP receptor protein. Biochem. J. 420, 413–420.