Difference between revisions of "Part:BBa K2960008:Design"

 
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===Design Notes===
 
===Design Notes===
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The mlrC gene sequence has been codon-optimized for expression in E. coli.  
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The mlrC gene sequence has been codon-optimized for expression in E. coli.
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+
  
 +
The construct was designed to be RFC[10] compatible.
  
 
===Source===
 
===Source===
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===References===
 
===References===
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Zhang, J., Lu, Q., Ding, Q., Yin, L., & Pu, Y. (2017). A Novel and Native Microcystin-Degrading Bacterium of Sphingopyxis sp. Isolated from Lake Taihu. International journal of environmental research and public health, 14(10), 1187. doi:10.3390/ijerph14101187
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Family M81. (n.d.). Retrieved from https://www.ebi.ac.uk/merops/cgi-bin/famsum?family=M81.
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Cerdà-Costa, N., & Gomis-Rüth, F. X. (2014). Architecture and function of metallopeptidase catalytic domains. Protein science : a publication of the Protein Society, 23(2), 123–144. doi:10.1002/pro.2400

Latest revision as of 01:16, 21 October 2019


The mlrC metalloprotease


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 549
    Illegal BglII site found at 759
    Illegal BamHI site found at 1294
    Illegal XhoI site found at 1038
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The mlrC gene sequence has been codon-optimized for expression in E. coli.

The construct was designed to be RFC[10] compatible.

Source

Sphingopyxis sp. C-1 genomic sequence. European Nucleotide Archive. (2008 October 22). Received from https://www.ebi.ac.uk/ena/data/view/AB468060

References

Zhang, J., Lu, Q., Ding, Q., Yin, L., & Pu, Y. (2017). A Novel and Native Microcystin-Degrading Bacterium of Sphingopyxis sp. Isolated from Lake Taihu. International journal of environmental research and public health, 14(10), 1187. doi:10.3390/ijerph14101187

Family M81. (n.d.). Retrieved from https://www.ebi.ac.uk/merops/cgi-bin/famsum?family=M81.

Cerdà-Costa, N., & Gomis-Rüth, F. X. (2014). Architecture and function of metallopeptidase catalytic domains. Protein science : a publication of the Protein Society, 23(2), 123–144. doi:10.1002/pro.2400