Difference between revisions of "Part:BBa K2960007:Design"
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The mlrB gene sequence has been codon-optimized for expression in E. coli. | The mlrB gene sequence has been codon-optimized for expression in E. coli. | ||
| − | + | The construct was designed to be RFC[10] compatible. | |
===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
| + | Zhang, J., Lu, Q., Ding, Q., Yin, L., & Pu, Y. (2017). A Novel and Native Microcystin-Degrading Bacterium of Sphingopyxis sp. Isolated from Lake Taihu. International journal of environmental research and public health, 14(10), 1187. doi:10.3390/ijerph14101187 | ||
Latest revision as of 01:15, 21 October 2019
The mlrB serine protease
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1568
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1124
Design Notes
The mlrB gene sequence has been codon-optimized for expression in E. coli.
The construct was designed to be RFC[10] compatible.
Source
Sphingopyxis sp. genomic sequence. European Nucleotide Archive. (2015 April 16). Received from https://www.ebi.ac.uk/ena/data/view/KR150744
References
Zhang, J., Lu, Q., Ding, Q., Yin, L., & Pu, Y. (2017). A Novel and Native Microcystin-Degrading Bacterium of Sphingopyxis sp. Isolated from Lake Taihu. International journal of environmental research and public health, 14(10), 1187. doi:10.3390/ijerph14101187