Difference between revisions of "Part:BBa K3105670"
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enhanced green-fluorescent protein codon optimised for <I>Pichia pastoris</I> without start-codon
 
enhanced green-fluorescent protein codon optimised for <I>Pichia pastoris</I> without start-codon
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<br><br>
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===Usage===
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eGFP was used as a reporter gene for assessing recombinant protein expression in <I>P. pastoris</I>. For this purpose, C-terminal fusions were created. Examples for this usage are the Parts <partinfo>BBa_K3105677</partinfo> and <partinfo>BBa_K3105680</partinfo>.
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<br>
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Functionality of this part in <i>P. pastoris</i> was shown in <partinfo>BBa_K3105680</partinfo>.
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===Usage and Biology===
 
===Usage and Biology===
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Latest revision as of 01:15, 21 October 2019


eGFP

enhanced green-fluorescent protein codon optimised for Pichia pastoris without start-codon

Usage

eGFP was used as a reporter gene for assessing recombinant protein expression in P. pastoris. For this purpose, C-terminal fusions were created. Examples for this usage are the Parts BBa_K3105677 and BBa_K3105680.
Functionality of this part in P. pastoris was shown in BBa_K3105680.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]