Difference between revisions of "Part:BBa K3105668"
 
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Coding sequence of horseradish peroxidase (HRP) C1A codon optimised for <I>Pichia pastoris</I>.
 
Coding sequence of horseradish peroxidase (HRP) C1A codon optimised for <I>Pichia pastoris</I>.
  
−
HRP is often used as a conjugate with antibodies and detected by chemiluminescence.  
+
HRP is commonly used as a antibody-conjugate and is detected by chemiluminescence.  
  
 
The underlying reaction mechanism is described below: <br>
 
The underlying reaction mechanism is described below: <br>
−
HRP+ H2O2 = oxidased substrate
+
HRP+ H<sub>2</sub>O<sub>2</sub> = oxidized substrate
  
 
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Latest revision as of 01:13, 21 October 2019


Horseradish peroxidase

Coding sequence of horseradish peroxidase (HRP) C1A codon optimised for Pichia pastoris.

HRP is commonly used as a antibody-conjugate and is detected by chemiluminescence.

The underlying reaction mechanism is described below:
HRP+ H2O2 = oxidized substrate


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 880
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 961
  • 1000
    COMPATIBLE WITH RFC[1000]