Difference between revisions of "Part:BBa K3105668"
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| − | Coding sequence of horseradish peroxidase (HRP) codon optimised for Pichia pastoris. | + | Coding sequence of horseradish peroxidase (HRP) C1A codon optimised for <I>Pichia pastoris</I>. |
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| + | HRP is commonly used as a antibody-conjugate and is detected by chemiluminescence. | ||
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| + | The underlying reaction mechanism is described below: <br> | ||
| + | HRP+ H<sub>2</sub>O<sub>2</sub> = oxidized substrate | ||
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| + | <br> | ||
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Latest revision as of 01:13, 21 October 2019
Horseradish peroxidase
Coding sequence of horseradish peroxidase (HRP) C1A codon optimised for Pichia pastoris.
HRP is commonly used as a antibody-conjugate and is detected by chemiluminescence.
The underlying reaction mechanism is described below:
HRP+ H2O2 = oxidized substrate
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 880
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 961
- 1000COMPATIBLE WITH RFC[1000]