Difference between revisions of "Part:BBa K3105667"
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<partinfo>BBa_K3105667 short</partinfo> | <partinfo>BBa_K3105667 short</partinfo> | ||
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Coding sequence of aryl-alcohol oxidase (AAO) (EC 1.1.3.7) codon optimised for <I>Pichia pastoris</I> | Coding sequence of aryl-alcohol oxidase (AAO) (EC 1.1.3.7) codon optimised for <I>Pichia pastoris</I> | ||
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AAO facilitates following reaction: | AAO facilitates following reaction: | ||
| − | primary aromatic alcohol + | + | primary aromatic alcohol + O<sub>2</sub> = aromatic aldehyde + H<sub>2</sub>O<sub>2</sub> |
<br><br><br> | <br><br><br> | ||
<b>References:</b> <br> | <b>References:</b> <br> | ||
Latest revision as of 01:13, 21 October 2019
Aryl-alcohol oxidase
Coding sequence of aryl-alcohol oxidase (AAO) (EC 1.1.3.7) codon optimised for Pichia pastoris
In wood-decaying fungi, AAO supports lignin degradation by providing peroxidases with the necessary hydrogenperoxide (Ferreira et al., 2009).
AAO facilitates following reaction:
primary aromatic alcohol + O2 = aromatic aldehyde + H2O2
References:
Ferreira, P. et al. (2009) ‘Aryl-alcohol oxidase involved in lignin degradation. A mechanistic study based on steady and pre-steady state kinetics and primary and solvent isotope effects with two alcohol substrates’, Journal of Biological Chemistry. doi: 10.1074/jbc.M109.011593.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 150
Illegal BamHI site found at 337 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1297
- 1000COMPATIBLE WITH RFC[1000]