Difference between revisions of "Part:BBa K3183006:Design"
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===Design Notes===
 
===Design Notes===
  
−
Codon optimized for <i>L. reuteri</i> using [http://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=1598 kazusa codon-usage tables.]<br>
+
Codon optimized for <i>E. coli</i> using [http://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=1598 kazusa codon-usage tables.]<br>
 
RF10, 12, 21, 23, 25, and 1000 Compatible
 
RF10, 12, 21, 23, 25, and 1000 Compatible
  
 
===Source===
 
===Source===
  
−
Derived from the <i>C. difficile</i> strain CD196 Genome; synthesized in IDT gBlocks.
+
Protein sequence from the <i>C. difficile</i> strain CD196 Genome; synthesized in IDT gBlocks.
  
 
===References===
 
===References===
 +
1. Darkoh, Charles, et al. “Toxin Synthesis by Clostridium Difficile Is Regulated through Quorum Signaling.” MBio, vol. 6, no. 2, May 2015, pp. e02569-14. mbio.asm.org, doi:10.1128/mBio.02569-14.

Latest revision as of 01:04, 21 October 2019


AgrB C. difficile Quorum Sensing Peptide Processing Protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for E. coli using [http://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=1598 kazusa codon-usage tables.]
RF10, 12, 21, 23, 25, and 1000 Compatible

Source

Protein sequence from the C. difficile strain CD196 Genome; synthesized in IDT gBlocks.

References

1. Darkoh, Charles, et al. “Toxin Synthesis by Clostridium Difficile Is Regulated through Quorum Signaling.” MBio, vol. 6, no. 2, May 2015, pp. e02569-14. mbio.asm.org, doi:10.1128/mBio.02569-14.