Difference between revisions of "Part:BBa K3183000:Design"
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<partinfo>BBa_K3183000 short</partinfo> | <partinfo>BBa_K3183000 short</partinfo> | ||
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| + | The part was identified in the article by Lizier et al; the gene was codon optimised for L. reuteri and synthesized by IDT. The part was leter assembled by Gibson Assembly into composite parts such as: BBa_K3183103, BBa_K3183100 etc., which were finally assembled into the pTRKH3 vector (BBa_K3183050), again by Gibson Assembly | ||
<partinfo>BBa_K3183000 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3183000 SequenceAndFeatures</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | This promoter was specifically selected as it conformed to the RF10, RF25, and | + | This promoter was specifically selected as an ideal promoter for <i> L. reuteri </i> because it conformed to the RF10, RF12, RF21, RF23, RF25, and RF1000 standards. |
===Source=== | ===Source=== | ||
| − | It is the erythromycin ribosomal methylase (ermB) promoter from the broad-host range plasmid pAMβ1 isolated from Enterococcus faecalis. We ordered the sequence as a gBlock from IDT. | + | It is the erythromycin ribosomal methylase (ermB) promoter from the broad-host range plasmid pAMβ1 isolated from <i>Enterococcus faecalis</i>. We ordered the sequence as a gBlock from IDT. |
===References=== | ===References=== | ||
| + | |||
| + | Lizier, Michela, et al. “Comparison of Expression Vectors in Lactobacillus Reuteri Strains.” FEMS Microbiology Letters, vol. 308, no. 1, Aug. 2010, pp. 8–15., doi:10.1111/j.1574-6968.2010.01978.x. | ||
Latest revision as of 00:31, 21 October 2019
Erythromycin Constitutive Promoter
The part was identified in the article by Lizier et al; the gene was codon optimised for L. reuteri and synthesized by IDT. The part was leter assembled by Gibson Assembly into composite parts such as: BBa_K3183103, BBa_K3183100 etc., which were finally assembled into the pTRKH3 vector (BBa_K3183050), again by Gibson Assembly
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This promoter was specifically selected as an ideal promoter for L. reuteri because it conformed to the RF10, RF12, RF21, RF23, RF25, and RF1000 standards.
Source
It is the erythromycin ribosomal methylase (ermB) promoter from the broad-host range plasmid pAMβ1 isolated from Enterococcus faecalis. We ordered the sequence as a gBlock from IDT.
References
Lizier, Michela, et al. “Comparison of Expression Vectors in Lactobacillus Reuteri Strains.” FEMS Microbiology Letters, vol. 308, no. 1, Aug. 2010, pp. 8–15., doi:10.1111/j.1574-6968.2010.01978.x.