Difference between revisions of "Part:BBa K3081053"
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Quorum sensing system is widely used in gene circuit design as a sensor of cell density. As a transcription factor, LuxR senses AHL and then activate the transcript of plux. In our project, we want dCas9 to express when bacteria density is high. So we put the dCas9 gene under the control of plux, and get this composite part. And we can use golden-gate assembly methods to change the sequence of sgRNA.  
 
Quorum sensing system is widely used in gene circuit design as a sensor of cell density. As a transcription factor, LuxR senses AHL and then activate the transcript of plux. In our project, we want dCas9 to express when bacteria density is high. So we put the dCas9 gene under the control of plux, and get this composite part. And we can use golden-gate assembly methods to change the sequence of sgRNA.  
  
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https://2019.igem.org/wiki/images/7/72/T--Peking--QS3.png
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https://2019.igem.org/wiki/images/b/b5/T--Peking--QS3-.png
 
Figure1 We transfer this part in <i>E. coli</i> ,BL21(DE3), and culture them in M9 medium containing a series of AHL concentration for 6 hours. Receiver cells show remarkable dose-dependent change in length with addition of AHL.  
 
Figure1 We transfer this part in <i>E. coli</i> ,BL21(DE3), and culture them in M9 medium containing a series of AHL concentration for 6 hours. Receiver cells show remarkable dose-dependent change in length with addition of AHL.  
  

Revision as of 00:07, 21 October 2019


AHL receiver device to expression of dCas9 and sgRNA

Quorum sensing system is widely used in gene circuit design as a sensor of cell density. As a transcription factor, LuxR senses AHL and then activate the transcript of plux. In our project, we want dCas9 to express when bacteria density is high. So we put the dCas9 gene under the control of plux, and get this composite part. And we can use golden-gate assembly methods to change the sequence of sgRNA.

T--Peking--QS3-.png Figure1 We transfer this part in E. coli ,BL21(DE3), and culture them in M9 medium containing a series of AHL concentration for 6 hours. Receiver cells show remarkable dose-dependent change in length with addition of AHL.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1321
    Illegal XhoI site found at 5538
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5728
    Illegal BsaI.rc site found at 1004
    Illegal BsaI.rc site found at 5324