Difference between revisions of "Part:BBa K3286200"

Revision as of 23:19, 20 October 2019

RpfCch

The RpfCch is a chimeric protein consisting of the RpfC X. fastidiosa N-terminal transmembrane domain (TMD) and the RpfC C-terminal histidine kinase part from X. campestris. This protein has been proven to sense X. fastidiosa DSF, as with the native X. fastidiosa RpfC, but now the requirement of another protein (X. fastidiosa RpfF) is not needed to sense DSF. The DSF sensing protein RpfC of X. campestris is already present in the repository under BBa_K1315003.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1861
Illegal PstI site found at 826
Illegal PstI site found at 1432
Illegal PstI site found at 2059
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1861
Illegal PstI site found at 826
Illegal PstI site found at 1432
Illegal PstI site found at 2059
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1861
Illegal BglII site found at 227
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1861
Illegal PstI site found at 826
Illegal PstI site found at 1432
Illegal PstI site found at 2059
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1861
Illegal PstI site found at 826
Illegal PstI site found at 1432
Illegal PstI site found at 2059
Illegal NgoMIV site found at 757
Illegal NgoMIV site found at 848
Illegal NgoMIV site found at 1675
Illegal NgoMIV site found at 1770
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3286200 short</partinfo>
-The RpfCch is a chimeric protein consisting of the RpfC X. fastidiosa N-terminal transmembrane domain (TMD) and the RpfC C-terminal histidine kinase part from X. campestris. This protein has been proven to sense X. fastidiosa DSF, as with the native X. fastidiosa RpfC, but now the requirement of another protein (X. fastidiosa RpfF) is not needed to sense DSF. The DSF sensing protein RpfC of X. campestris is already present in the repository under <partinfo>BBa_K1315003</partinfo>.
+The RpfCch is a chimeric protein consisting of the RpfC <i>X. fastidiosa</i> N-terminal transmembrane domain (TMD) and the RpfC C-terminal histidine kinase part from <i>X. campestris</i>. This protein has been proven to sense <i>X. fastidiosa</i> DSF, as with the native <i>X. fastidiosa</i> RpfC, but now the requirement of another protein (<i>X. fastidiosa</i> RpfF) is not needed to sense DSF. The DSF sensing protein RpfC of <i>X. campestris</i> is already present in the repository under <partinfo>BBa_K1315003</partinfo>.
 <!-- Add more about the biology of this part here