Difference between revisions of "Part:BBa K3059620"
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The native curli operon in MG1655 E. coli contains two divergent operons: csgBAC as well as csgDEFG. To create a synthetic curli operon, both csgBAC and csgEFG are necessary (for example, though csgA is the main subunit of the eventual fiber, csgG is responsible for fiber export out of the cell). csgD regulates csgBAC, and is thus unnecessary in a synthetic operon where both component operons are combined under the control of one promoter. | The native curli operon in MG1655 E. coli contains two divergent operons: csgBAC as well as csgDEFG. To create a synthetic curli operon, both csgBAC and csgEFG are necessary (for example, though csgA is the main subunit of the eventual fiber, csgG is responsible for fiber export out of the cell). csgD regulates csgBAC, and is thus unnecessary in a synthetic operon where both component operons are combined under the control of one promoter. | ||
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| + | <img src="https://2019.igem.org/wiki/images/2/2b/T--William_and_Mary--wildvssynthcurliJ.jpeg" width="800px" class="center"/> | ||
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When successfully assembled in a transcriptional unit with a promoter, csgBAC, csgEFG, and a terminator, this RBS results in curli amyloid fiber production. Curli fibers constitute the main proteinaceous component of Enterobacteriaceae species such as E. coli (Barnhart & Chapman, 2006). | When successfully assembled in a transcriptional unit with a promoter, csgBAC, csgEFG, and a terminator, this RBS results in curli amyloid fiber production. Curli fibers constitute the main proteinaceous component of Enterobacteriaceae species such as E. coli (Barnhart & Chapman, 2006). | ||
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| + | <img src="https://2019.igem.org/wiki/images/5/58/T--William_and_Mary--curlioperonTU.jpeg" width="750px" class="center"/> | ||
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| + | Proposed transcriptional unit with this part. For a 3G-compatible version of this part (and more information on construction of a complete synthetic curli operon), see BBa_K3059629. | ||
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Latest revision as of 23:03, 20 October 2019
pre-csgE RBS
RBS to follow csgBAC and precede csgEFG operon in a synthetic curli operon.
The native curli operon in MG1655 E. coli contains two divergent operons: csgBAC as well as csgDEFG. To create a synthetic curli operon, both csgBAC and csgEFG are necessary (for example, though csgA is the main subunit of the eventual fiber, csgG is responsible for fiber export out of the cell). csgD regulates csgBAC, and is thus unnecessary in a synthetic operon where both component operons are combined under the control of one promoter.
When successfully assembled in a transcriptional unit with a promoter, csgBAC, csgEFG, and a terminator, this RBS results in curli amyloid fiber production. Curli fibers constitute the main proteinaceous component of Enterobacteriaceae species such as E. coli (Barnhart & Chapman, 2006).
Proposed transcriptional unit with this part. For a 3G-compatible version of this part (and more information on construction of a complete synthetic curli operon), see BBa_K3059629.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]