Difference between revisions of "Part:BBa K2978211"
 
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<h2>Terminator for <i>Lactobacillus spp</i></h2>
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===Description===
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* The <i>in silico</i> sequence was obtained from the Duong <i>et al</i> (2010) where they tested this Rho-independent terminator and the [[Part:BBa_K2978212| K2978212]] terminator.
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*This terminator determined with a dyad symmetry analysis and then was selectioned according the lowest values of Gibbs Free energy, this in specific had D°G=-23.5 kcal mol-1 which represents to be an optimal low entrophic value for terminators (Duong <i> et al</i> 2010).
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*The authors of this paper suggests that this specific terminator had the capability to be used as a bidirectional terminator.
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* The composite part of this sequence is present in [[Part:BBa_K2978203| K2978203]]
 
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[[Image:Terminator.png]]
 
[[Image:Terminator.png]]
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<partinfo>BBa_K2978211 parameters</partinfo>
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<partinfo>BBa_K2978211 short</partinfo>
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* The <i>in silico</i> sequence was obtained from the Duong <i>et al</i> (2010) where they tested this Rho-independent terminator and the [[Part:BBa_K2978212| K2978212]] terminator.
+
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*This terminator was identified with a dyad symmetry analysis and then was selectioned according the lowest values of Gibbs Free energy, this in specific had D°G=-24.4 kcal mol-1 which represents to be an optimal low entrophic value for terminators (Duong <i> et al</i> 2010).
+
−
*The authors of this paper suggests that this specific terminator had the capability to be used as a bidirectional terminator.
+
−
* The composite part of this sequence is present in [[Part:BBa_K2978203| K2978203]]
+
 
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Latest revision as of 22:02, 20 October 2019


Terminator for Lactobacillus spp

Description

  • The in silico sequence was obtained from the Duong et al (2010) where they tested this Rho-independent terminator and the K2978212 terminator.
  • This terminator determined with a dyad symmetry analysis and then was selectioned according the lowest values of Gibbs Free energy, this in specific had D°G=-23.5 kcal mol-1 which represents to be an optimal low entrophic value for terminators (Duong et al 2010).
  • The authors of this paper suggests that this specific terminator had the capability to be used as a bidirectional terminator.
  • The composite part of this sequence is present in K2978203

Terminator.png


Secondary Structure

Mfold-K2978211-1.png


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]