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This BioBrick is part of the measurement entry vectors for the characterization of BioBricks in cyanobacteria (Part range <a href="https://parts.igem.org/Part:BBa_K3228073"> BBa_K3228073</a> to <a href="https://parts.igem.org/Part:BBa_K3228075"> BBa_K3228075</a> as well as <a href="https://parts.igem.org/Part:BBa_K3228090"> BBa_K3228090</a>) by the iGEM Team Marburg 2019. They contain our MoClo compatible shuttle vector for cyanobacteria <a href="https://parts.igem.org/Part:BBa_K3228069"> BBa_K3228069</a> and are therefore the only MoClo based vectors for the characterization of BioBricks in cyanobacteria. These pre assembled LVL 1 plasmids contain a place holder <a href="https://parts.igem.org/Part:BBa_K3228060"> (compare BBa_K3228060)</a> for their respective BioBrick type that acts as a Dropout to quickly and effortlessly insert any part of the same type for an easy characterization.
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Picture of the promoter and rbs entry vectors for measurement.
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<div style="width:100%;display:flex;flex-direction:row;flex-wrap: wrap; justify-content:space-evenly; align-items:center;">[[File:GE LVL1 measurementvectors.png|500px|thumb|left|'''Figure 1''': Design of the standardized measurement vectors for promoter and RBS BioBrick characterization ]]
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Picture of the LVL 2 entry vector for measuring terminators (with YFP and mTurqoise)
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In our <a href="https://2019.igem.org/Team:Marburg/Results">results</a>we show how these measurement entry vectors can save a lot of effort and money when characterizing a greater library of parts. Additionally the usage of the same entry vector for each measurement will aid in greater comparability and reproducibility.
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This BioBrick is part of the measurement entry vectors for the characterization of BioBricks in cyanobacteria (Part range <a href="https://parts.igem.org/Part:BBa_K3228073"> BBa_K3228073</a> to <a href="https://parts.igem.org/Part:BBa_K3228075"> BBa_K3228075</a> as well as <a href="https://parts.igem.org/Part:BBa_K3228090"> BBa_K3228090</a>) by the iGEM Team Marburg 2019. They contain our MoClo compatible shuttle vector for cyanobacteria <a href="https://parts.igem.org/Part:BBa_K3228069"> BBa_K3228069</a> and are therefore the only MoClo based vectors for the characterization of BioBricks in cyanobacteria. These pre assembled LVL 1 plasmids contain a place holder for their respective BioBrick type that acts as a Dropout to quickly and effortlessly insert any part of the same type for an easy characterization. In our <a href="https://2019.igem.org/Team:Marburg/Results">results</a>we show how these measurement entry vectors can save a lot of effort and money when characterizing a greater library of parts. Additionally the usage of the same entry vector for each measurement will aid in greater comparability and reproducibility.
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For greater comparability across other data sets we decided to use the same Biobricks as in measuring the toolbox for <a href="http://2018.igem.org/Team:Marburg/Results"> Vibrio natriegens in the last year</a>.
For greater comparability across other data sets we decided to use the same Biobricks as in measuring the toolbox for <a href="http://2018.igem.org/Team:Marburg/Results"> Vibrio natriegens in the last year</a>.
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These parts are compatible with the RCF [1000] standard. These parts are compatible with any Part Collection that uses the Phytobrick standard of overhangs. For more information we recommend to head over to <a href="http://2018.igem.org/Team:Marburg/Design">Design of the Marburg Collection</a>.
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These parts are compatible with the RCF [1000] standard and can be used in any part collection that uses the PhytoBrick standard of overhangs. For more information we recommend to head over to <a href="http://2018.igem.org/Team:Marburg/Design">Design of the Marburg Collection</a>.
Illegal XbaI site found at 3503 Illegal PstI site found at 6038
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INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 6038 Illegal NotI site found at 2305 Illegal NotI site found at 8042
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INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 7201 Illegal XhoI site found at 3553
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INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 3503 Illegal PstI site found at 6038
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INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 3503 Illegal PstI site found at 6038 Illegal NgoMIV site found at 2126 Illegal NgoMIV site found at 2352 Illegal NgoMIV site found at 2494
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COMPATIBLE WITH RFC[1000]
Description
This BioBrick is part of the measurement entry vectors for the characterization of BioBricks in cyanobacteria (Part range BBa_K3228073 to BBa_K3228075 as well as BBa_K3228090) by the iGEM Team Marburg 2019. They contain our MoClo compatible shuttle vector for cyanobacteria BBa_K3228069 and are therefore the only MoClo based vectors for the characterization of BioBricks in cyanobacteria. These pre assembled LVL 1 plasmids contain a place holder (compare BBa_K3228060) for their respective BioBrick type that acts as a Dropout to quickly and effortlessly insert any part of the same type for an easy characterization.
Figure 1: Design of the standardized measurement vectors for promoter and RBS BioBrick characterization
In our resultswe show how these measurement entry vectors can save a lot of effort and money when characterizing a greater library of parts. Additionally the usage of the same entry vector for each measurement will aid in greater comparability and reproducibility.
For greater comparability across other data sets we decided to use the same Biobricks as in measuring the toolbox for Vibrio natriegens in the last year.
Compability
These parts are compatible with the RCF [1000] standard and can be used in any part collection that uses the PhytoBrick standard of overhangs. For more information we recommend to head over to Design of the Marburg Collection.
