Difference between revisions of "Part:BBa K3009012"
 
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Racemases exist in many organisms, especially bacteria, and convert L- to D-amino acids for different purposes, like building the bacterial cell wall or using D-amino acids as a neurotransmitter.
 
Racemases exist in many organisms, especially bacteria, and convert L- to D-amino acids for different purposes, like building the bacterial cell wall or using D-amino acids as a neurotransmitter.
 
The aspartate racemase is consequently converting L-aspartate to D-aspartate.
 
The aspartate racemase is consequently converting L-aspartate to D-aspartate.
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We purified it via his-tag purification to perform further experiments.
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<html>
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<figure>
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<img style="width:50%;" src="https://2019.igem.org/wiki/images/f/fc/T--Freiburg--racemase_purification.png">
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<figcaption>
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<b> Fig.2:</b> His-Tag Purification of <i>P.horikoshii</i> aspartate racemase.
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            Bands are clearly visible at 40 kDA.
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</figcaption>
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</figure>
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</html>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 21:01, 20 October 2019


Aspartate Racemase (PhAspR)

Racemases exist in many organisms, especially bacteria, and convert L- to D-amino acids for different purposes, like building the bacterial cell wall or using D-amino acids as a neurotransmitter. The aspartate racemase is consequently converting L-aspartate to D-aspartate.


We purified it via his-tag purification to perform further experiments.

Fig.2: His-Tag Purification of P.horikoshii aspartate racemase. Bands are clearly visible at 40 kDA.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 363
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]