Difference between revisions of "Part:BBa K2940014:Design"

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===Design Notes===
 
===Design Notes===
  
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    The part is identical to BBa_K2023015 part developed by Ionis Paris iGEM team 2016 [1], except for the originally used wild-type GFP (BBa_E0040) reporter gene being replaced by codon-optimized superfolder GFP (BBa_K2541400).
+
The part is identical to BBa_K2023015 part developed by Ionis Paris iGEM team 2016 [1], except for the originally used wild-type GFP (BBa_E0040) reporter gene being replaced by codon-optimized superfolder GFP (BBa_K2541400).
  
−
    The codon-optimized superfolder GFP was used as the reporter in an attempt to have a very sensitive starting biosensor genetic circuit for the subsequent directed evolution and screenings for the biosensors with new substrate specificities.
+
The codon-optimized superfolder GFP was used as the reporter in an attempt to have a very sensitive starting biosensor genetic circuit for the subsequent directed evolution and screenings for the biosensors with new substrate specificities.
  
 
===Source===
 
===Source===

Revision as of 20:53, 20 October 2019


XylR coding device-sfGFP_optimism coding device with Pu


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2446
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 329
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is identical to BBa_K2023015 part developed by Ionis Paris iGEM team 2016 [1], except for the originally used wild-type GFP (BBa_E0040) reporter gene being replaced by codon-optimized superfolder GFP (BBa_K2541400).

The codon-optimized superfolder GFP was used as the reporter in an attempt to have a very sensitive starting biosensor genetic circuit for the subsequent directed evolution and screenings for the biosensors with new substrate specificities.

Source

The part is identical to BBa_K2023015 part developed by Ionis Paris iGEM team 2016, except for the originally used wild-type GFP (BBa_E0040) reporter gene being replaced by codon-optimized superfolder GFP (BBa_K2541400).

References

[1] http://2016.igem.org/Team:Ionis_Paris