Difference between revisions of "Part:BBa K2940003"

Revision as of 20:33, 20 October 2019

Synthetic silk MaSp1 with flanking solubilizing blocks and head-to-tail assembly system

Silk proteins are composed of long stretches of monomer repeats. Major Ampullate Spidroin (MaSp1) protein is a monomer component of dragline spider silk. This BioBrick encodes a single monomer of MaSp1 methionine added with a system in place to build monomer repeats using repeated digestion and ligation (head-to-tail multimerization). This allows a simple way to build long chains of MaSp1 repeats to produce the silk protein fiber. The BioBrick also contains a polyglutamine sequence flanking the monomer repeats motif which helps to solubilize the produced protein.

Usage and Biology

• N. clavipes truncated version of MaSp1 monomer repeat for synthetic silk production.

• Head-to-tail multimerization strategy to create long monomer repeat sequences by doubling the size.

• Flanking glutamic blocks for solubilization

Characterization

Sequence and Features