Difference between revisions of "Part:BBa K2940003"

(Usage and Biology)
Line 7: Line 7:
 
===Usage and Biology===
 
===Usage and Biology===
  
• N. clavipes truncated version of MaSp1 monomer repeat for synthetic silk production
+
• N. clavipes truncated version of MaSp1 monomer repeat for synthetic silk production.
• Head-to-tail multimerization strategy to create long monomer repeat sequences by doubling the size.
+
 
• Flanking glutamic blocks for solubilization
+
• Head-to-tail multimerization strategy to create long monomer repeat sequences by doubling the size.
 +
 
 +
• Flanking glutamic blocks for solubilization
  
  

Revision as of 20:29, 20 October 2019


Synthetic silk MaSp1 with flanking solubilizing blocks and head-to-tail assembly system

Silk proteins are composed of long stretches of monomer repeats. Major Ampullate Spidroin (MaSp1) protein is a monomer component of dragline spider silk. This BioBrick encodes a single monomer of MaSp1 methionine added with a system in place to build monomer repeats using repeated digestion and ligation (head-to-tail multimerization). This allows a simple way to build long chains of MaSp1 repeats to produce the silk protein fiber. The BioBrick also contains a polyglutamine sequence flanking the monomer repeats motif which helps to solubilize the produced protein.

Usage and Biology

• N. clavipes truncated version of MaSp1 monomer repeat for synthetic silk production.

• Head-to-tail multimerization strategy to create long monomer repeat sequences by doubling the size.

• Flanking glutamic blocks for solubilization


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 128
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 20
    Illegal SpeI site found at 128
    Illegal NotI site found at 27
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 128
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 128
  • 1000
    COMPATIBLE WITH RFC[1000]