Difference between revisions of "Part:BBa K2940003"
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Silk proteins are composed of long stretches of monomer repeats. Major Ampullate Spidroin (MaSp1) protein is a monomer component of dragline spider silk. This BioBrick encodes a single monomer of MaSp1 methionine added with a system in place to build monomer repeats using repeated digestion and ligation (head-to-tail multimerization). This allows a simple way to build long chains of MaSp1 repeats to produce the silk protein fiber. The BioBrick also contains a polyglutamine sequence flanking the monomer repeats motif which helps to solubilize the produced protein. | Silk proteins are composed of long stretches of monomer repeats. Major Ampullate Spidroin (MaSp1) protein is a monomer component of dragline spider silk. This BioBrick encodes a single monomer of MaSp1 methionine added with a system in place to build monomer repeats using repeated digestion and ligation (head-to-tail multimerization). This allows a simple way to build long chains of MaSp1 repeats to produce the silk protein fiber. The BioBrick also contains a polyglutamine sequence flanking the monomer repeats motif which helps to solubilize the produced protein. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 20:29, 20 October 2019
Synthetic silk MaSp1 with flanking solubilizing blocks and head-to-tail assembly system
Silk proteins are composed of long stretches of monomer repeats. Major Ampullate Spidroin (MaSp1) protein is a monomer component of dragline spider silk. This BioBrick encodes a single monomer of MaSp1 methionine added with a system in place to build monomer repeats using repeated digestion and ligation (head-to-tail multimerization). This allows a simple way to build long chains of MaSp1 repeats to produce the silk protein fiber. The BioBrick also contains a polyglutamine sequence flanking the monomer repeats motif which helps to solubilize the produced protein.
Usage and Biology
• N. clavipes truncated version of MaSp1 monomer repeat for synthetic silk production • Head-to-tail multimerization strategy to create long monomer repeat sequences by doubling the size. • Flanking glutamic blocks for solubilization
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 128
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 20
Illegal SpeI site found at 128
Illegal NotI site found at 27 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 128
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 128
- 1000COMPATIBLE WITH RFC[1000]