Difference between revisions of "Part:BBa K2924022:Design"
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===Design Notes===
 
===Design Notes===
−
The genes from <i>E. Coli K12</> were cloned into pet21a in an operon-like manner to ensure transcription of all genes of the complex at the same level and from the same vector.
+
The gene from <i>E. Coli K12</> was cloned into pet21a in an operon-like manner to ensure transcription of all genes of the complex at the same level and from the same vector.
  
  
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===Source===
 
===Source===
  
−
The gene were cloned from the genome of <i>E. coli K12</i>
+
The gene was cloned from the genome of <i>E. coli K12</i>
  
 
===References===
 
===References===

Revision as of 19:42, 20 October 2019


accA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 579
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 517
    Illegal SapI.rc site found at 714


Design Notes

The gene from E. Coli K12</> was cloned into pet21a in an operon-like manner to ensure transcription of all genes of the complex at the same level and from the same vector.



Source

The gene was cloned from the genome of <i>E. coli K12

References