Difference between revisions of "Part:BBa K3037009"
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|'''Backbone'''
 
|'''Backbone'''
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|pSB1C3<br>  
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|[https://parts.igem.org/Help:2019_DNA_Distribution pSB1C3<br>]
 
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|'''Submitted by'''
 
|'''Submitted by'''

Revision as of 18:49, 20 October 2019

HRP+Strep-tag

HRP+Strep-tag
Function Expression
Use in Escherichia coli
RFC standard Freiburg RFC25 standard
Backbone pSB1C3
Submitted by Team: TU_Dresden 2019


Overview

The TU Dresden 2019 team design this biobrick using the BBa_K823038 (more information).

HRP+Strep-tag was inserted into the pSB1C3 vector for transformation and expressed in Escherichia coli.

https://parts.igem.org/Assembly_standard_25

The HRP was made from BBa_K1800002 but adapted to the RFC 25 standard

Sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 151
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 393
    Illegal XhoI site found at 477
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]