Difference between revisions of "Part:BBa K2940003"

Revision as of 18:45, 20 October 2019

Synthetic silk MaSp1 with flanking solubilizing blocks and head-to-tail assembly system

Silk proteins are composed of long stretches of monomer repeats. Major Ampullate Spidroin (MaSp1) protein is a monomer component of dragline spider silk. This BioBrick encodes a single monomer of MaSp1 methionine added with a system in place to build monomer repeats using repeated digestion and ligation (head-to-tail multimerization). This allows a simple way to build long chains of MaSp1 repeats to produce the silk protein fiber. The BioBrick also contains a polyglutamine sequence flanking the monomer repeats motif which helps to solubilize the produced protein.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 128
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 20
Illegal SpeI site found at 128
Illegal NotI site found at 27
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 128
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 128
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2940003 short</partinfo>
-Ready to use Golden Gate multimerization assembly to create synthetic silk MaSp1 monomer repeats. Flanking the MaSp1 gene, five glutamic acids had been added as solubilizing blocks. The composite presents orthogonality since the MaSp1 can be changed for any other part desired to create monomer repeats.
+Silk proteins are composed of long stretches of monomer repeats. Major Ampullate Spidroin (MaSp1) protein is a monomer component of dragline spider silk. This BioBrick encodes a single monomer of MaSp1 methionine added with a system in place to build monomer repeats using repeated digestion and ligation (head-to-tail multimerization). This allows a simple way to build long chains of MaSp1 repeats to produce the silk protein fiber. The BioBrick also contains a polyglutamine sequence flanking the monomer repeats motif which helps to solubilize the produced protein.
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