Difference between revisions of "Part:BBa K137132"
 
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<partinfo>BBa_K137132 short</partinfo>
 
<partinfo>BBa_K137132 short</partinfo>
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A test construct to measure the response of the lactose-inducible promoter B4 with and without the expression of the mutant LacY permease K137004. In the final system design, GFP will be replaced by the lysis cassette (K137126) making K137131.
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A test construct to measure the response of the lactose-inducible [https://parts.igem.org/Part:BBa_K137125 promoter B4] with and without the expression of the mutant [https://parts.igem.org/Part:BBa_K137004 LacY permease]. In the final system design, GFP will be replaced by the lysis cassette [https://parts.igem.org/Part:BBa_K137126 K137126] making [https://parts.igem.org/Part:BBa_K137131 K137131].
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 19:39, 23 October 2008

Lactose inducible GFP expression

A test construct to measure the response of the lactose-inducible promoter B4 with and without the expression of the mutant LacY permease. In the final system design, GFP will be replaced by the lysis cassette K137126 making K137131.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1208
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2132