Difference between revisions of "Part:BBa K3171177"

Revision as of 18:08, 20 October 2019

HisD 3000bp Homologous part 1

We experimented with CRISPR with the aim to make a histidine auxotroph by knocking out HisD in both E. coli and V. natriegens. To make a histidine auxotroph by knocking out HisD in E. coli specific gRNA targets were selected for knockout of the HisD gene. We designed 3 homologous parts Part:BBa_K3171177, Part:BBa_K3171178, Part:BBa_K3171179 in order to decide on the correct homologous part required for knockout. 3 gRNA target sites Part:BBa_K3171180, Part:BBa_K3171181, Part:BBa_K3171182 were selected for knock out to determine the exact region for knockout.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 689
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1472
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 689
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 689
Illegal AgeI site found at 1885
Illegal AgeI site found at 2788
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 807

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3171177 short</partinfo>
-We experimented with CRISPR with the aim to make a histidine auxotroph by knocking out HisD in both E. coli and V. natriegens. To make a histidine auxotroph by knocking out HisD in E. coli specific gRNA targets were selected for knockout of the HisD gene. We designed 3 homologous parts [[Part:BBa_K3171177]], [[Part:BBa_K3171178]], [[Part:BBa_K3171179]] in order to decide on the correct homologous part required for knockout. 3 gRNA target sites [[Part:BBa_K3171180]], [[Part:BBa_K3171181]], [[Part:BBa_K3171182]] were selected for knock out.
+We experimented with CRISPR with the aim to make a histidine auxotroph by knocking out HisD in both E. coli and V. natriegens. To make a histidine auxotroph by knocking out HisD in E. coli specific gRNA targets were selected for knockout of the HisD gene. We designed 3 homologous parts [[Part:BBa_K3171177]], [[Part:BBa_K3171178]], [[Part:BBa_K3171179]] in order to decide on the correct homologous part required for knockout. 3 gRNA target sites [[Part:BBa_K3171180]], [[Part:BBa_K3171181]], [[Part:BBa_K3171182]] were selected for knock out to determine the exact region for knockout.
 <!-- Add more about the biology of this part here