Difference between revisions of "Part:BBa K2984046"

Latest revision as of 17:57, 20 October 2019

PsadIntron - Promoter for enhanced expression - A1-A3

This part was designed with the AR promoter as a model. The AR Promoter is composed of the HSP70 promoter fused to the RbcS2 intron. We followed a similar approach by fusing the RbcS2 intron to the end of Psad promoter , hoping to achieve a higher expression yield. Every CDS assembled with this part is then fused to the RbcS2 intron. This part is a part of the Chlamy-HUB-Collection.It is desgined according to MoClo standard carrying A1-A3 overhangs.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 19
Illegal XhoI site found at 997
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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-This part was desgined with the <a href=""> AR promoter </a> as model. The AR Promoter is composed of the HSP70 promoter fused to the RbcS2 intron. We followed a similar approach by fusing the RbcS2 intron to the end of <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008"> Psad promoter </a>, hoping to achieve a higher expression yield. Every CDS assembled with this part is then fused to the RbcS2 intron.
+This part was designed with the <a href=""> AR promoter </a> as a model. The AR Promoter is composed of the HSP70 promoter fused to the RbcS2 intron. We followed a similar approach by fusing the RbcS2 intron to the end of <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008"> Psad promoter </a>, hoping to achieve a higher expression yield. Every CDS assembled with this part is then fused to the RbcS2 intron.
 This part is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>.It is desgined according to MoClo standard carrying A1-A3 overhangs.
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