Difference between revisions of "Part:BBa K2984046"
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| − | This part was | + | This part was designed with the <a href=""> AR promoter </a> as a model. The AR Promoter is composed of the HSP70 promoter fused to the RbcS2 intron. We followed a similar approach by fusing the RbcS2 intron to the end of <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008"> Psad promoter </a>, hoping to achieve a higher expression yield. Every CDS assembled with this part is then fused to the RbcS2 intron. |
| − | This part is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>.It is desgined according to MoClo standard | + | This part is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>.It is desgined according to MoClo standard carrying A1-A3 overhangs. |
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Latest revision as of 17:57, 20 October 2019
PsadIntron - Promoter for enhanced expression - A1-A3
This part was designed with the AR promoter as a model. The AR Promoter is composed of the HSP70 promoter fused to the RbcS2 intron. We followed a similar approach by fusing the RbcS2 intron to the end of Psad promoter , hoping to achieve a higher expression yield. Every CDS assembled with this part is then fused to the RbcS2 intron. This part is a part of the Chlamy-HUB-Collection.It is desgined according to MoClo standard carrying A1-A3 overhangs.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 19
Illegal XhoI site found at 997 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]