Difference between revisions of "Part:BBa K2984046"

 
 
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<partinfo>BBa_K2984046 short</partinfo>
 
<partinfo>BBa_K2984046 short</partinfo>
  
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Psad Promoter fused to Rbcs2 Intron
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This part was designed with the <a href=""> AR promoter </a> as a model. The AR Promoter is composed of the HSP70 promoter fused to the RbcS2 intron. We followed a similar approach by fusing the RbcS2 intron to the end of <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008"> Psad promoter </a>, hoping to achieve a higher expression yield. Every CDS assembled with this part is then fused to the RbcS2 intron.
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This part is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>.It is desgined according to MoClo standard carrying A1-A3 overhangs.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 17:57, 20 October 2019


PsadIntron - Promoter for enhanced expression - A1-A3

This part was designed with the AR promoter as a model. The AR Promoter is composed of the HSP70 promoter fused to the RbcS2 intron. We followed a similar approach by fusing the RbcS2 intron to the end of Psad promoter , hoping to achieve a higher expression yield. Every CDS assembled with this part is then fused to the RbcS2 intron. This part is a part of the Chlamy-HUB-Collection.It is desgined according to MoClo standard carrying A1-A3 overhangs.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 19
    Illegal XhoI site found at 997
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]