Difference between revisions of "Part:BBa K2923021"
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− | Our bacterial three-hybrid (BH3) construct (inspired by Berry and Hochschild, 2018) is composed of two fusion proteins with MS2 and PP7 coat | + | Our bacterial three-hybrid (BH3) construct (inspired by Berry and Hochschild, 2018) is composed of two fusion proteins with MS2 and PP7 coat proteins and RNA. To make the interaction between BH3 and guanine aptazyme iGEM Strasbourg created an aptazyme bordered by MS2 and PP7 stem-loops. This sequence corresponds to inactive aptazyme: the ribozyme module is mutated and the aptazyme lost self-cleavage capability. iGEM Strasbourg 2019 used it as a negative control for the part: <partinfo>BBa_K2923020</partinfo> |
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+ | [[Image: tout Ms2-MS2-Aptazyme-PP7-PP7.jpg|500px|thumb|center| Figure 1: Plasmid overview of MS2-MS2-Aptazyme-PP7-PP7 | ||
+ | ]] | ||
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+ | This sequence corresponds to inactive aptazyme: the ribozyme module is mutated and the aptazyme lost self-cleavage capability. iGEM Strasbourg 2019 used it as a negative control for the part: <partinfo>BBa_K2923020</partinfo> | ||
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+ | [[Image: Aptazyme inactive process.jpg|500px|thumb|center| Figure 2: Schematic graphic of inactive aptazyme. Inactive aptazymes (lost self-cleavage capability) active BH3 system independently of ligand presence | ||
+ | ]] | ||
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Latest revision as of 17:19, 20 October 2019
MS2-MS2-Guanine inactif aptazyme-PP7-PP7
Our bacterial three-hybrid (BH3) construct (inspired by Berry and Hochschild, 2018) is composed of two fusion proteins with MS2 and PP7 coat proteins and RNA. To make the interaction between BH3 and guanine aptazyme iGEM Strasbourg created an aptazyme bordered by MS2 and PP7 stem-loops. This sequence corresponds to inactive aptazyme: the ribozyme module is mutated and the aptazyme lost self-cleavage capability. iGEM Strasbourg 2019 used it as a negative control for the part: BBa_K2923020
This sequence corresponds to inactive aptazyme: the ribozyme module is mutated and the aptazyme lost self-cleavage capability. iGEM Strasbourg 2019 used it as a negative control for the part: BBa_K2923020
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 37
Illegal PstI site found at 25
Illegal PstI site found at 64 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 25
Illegal PstI site found at 64 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 37
Illegal PstI site found at 25
Illegal PstI site found at 64 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 37
Illegal PstI site found at 25
Illegal PstI site found at 64 - 1000COMPATIBLE WITH RFC[1000]