Difference between revisions of "Part:BBa K2923001"
 
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An aptazyme is an aptamer-ribozyme combination with intrinsic RNA cleavage activity. This aptazyme sequence is specific for the theophylline (ligand).  
 
An aptazyme is an aptamer-ribozyme combination with intrinsic RNA cleavage activity. This aptazyme sequence is specific for the theophylline (ligand).  
 
Theophylline (1,3-dimethylxanthine) is a drug used in therapy for respiratory diseases and is structurally similar to theobromine and caffeine.  
 
Theophylline (1,3-dimethylxanthine) is a drug used in therapy for respiratory diseases and is structurally similar to theobromine and caffeine.  
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Aptazyme is composed of 3 different parts, a twister ribozyme module, a communication module, and an aptamer module.
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Aptazyme is composed of 3 different parts: a twister ribozyme module, a communication module and an aptamer module.
  
 
[[Image: BasicPartsimg1.jpg|400px|thumb|center|Figure 1: Theophylline aptazyme secondary structure. The Shine-Dalgarno (SD) and the anti-SD are shown in light blue and grey, respectively. The inactivating mutation is highlighted in yellow. The cleavage site is indicated with a grey arrow. The figure is reproduced from [Stifel, J., Spöring, M., and Hartig, J.S. (2019). Expanding the toolbox of synthetic riboswitches with guanine-dependent aptazymes. Synthetic Biology 4]]
 
[[Image: BasicPartsimg1.jpg|400px|thumb|center|Figure 1: Theophylline aptazyme secondary structure. The Shine-Dalgarno (SD) and the anti-SD are shown in light blue and grey, respectively. The inactivating mutation is highlighted in yellow. The cleavage site is indicated with a grey arrow. The figure is reproduced from [Stifel, J., Spöring, M., and Hartig, J.S. (2019). Expanding the toolbox of synthetic riboswitches with guanine-dependent aptazymes. Synthetic Biology 4]]
  
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The described anti-theophylline aptazyme is an OFF-switch. Its catalytic activity (self-cleavage) requires the absence of ligand.  
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The described anti-theophylline aptazyme is an switch-off. Its catalytic activity (self-cleavage) requires the absence of ligand.  
  
 
[[Image: Aptazyme off process.jpg|500px|thumb|center| Figure 2: Schematic graphic of Aptazyme switch-off catalytic activity]]
 
[[Image: Aptazyme off process.jpg|500px|thumb|center| Figure 2: Schematic graphic of Aptazyme switch-off catalytic activity]]

Latest revision as of 16:59, 20 October 2019


Theophylline actif aptazyme

An aptazyme is an aptamer-ribozyme combination with intrinsic RNA cleavage activity. This aptazyme sequence is specific for the theophylline (ligand). Theophylline (1,3-dimethylxanthine) is a drug used in therapy for respiratory diseases and is structurally similar to theobromine and caffeine. Aptazyme is composed of 3 different parts: a twister ribozyme module, a communication module and an aptamer module.

Figure 1: Theophylline aptazyme secondary structure. The Shine-Dalgarno (SD) and the anti-SD are shown in light blue and grey, respectively. The inactivating mutation is highlighted in yellow. The cleavage site is indicated with a grey arrow. The figure is reproduced from [Stifel, J., Spöring, M., and Hartig, J.S. (2019). Expanding the toolbox of synthetic riboswitches with guanine-dependent aptazymes. Synthetic Biology 4

The described anti-theophylline aptazyme is an switch-off. Its catalytic activity (self-cleavage) requires the absence of ligand.

Figure 2: Schematic graphic of Aptazyme switch-off catalytic activity


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]