Difference between revisions of "Part:BBa K3142003"
 
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<partinfo>BBa_K3142003 short</partinfo>
 
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nisi&#25239;&#24615;&#22522;&#22240;&#65292;&#26159;&#19968;&#20010;&#39135;&#21697;&#32423;&#30340;&#25239;&#24615;&#31579;&#36873;&#22522;&#22240;&#65292;&#21487;&#20197;&#29992;&#26469;&#26500;&#24314;&#39135;&#21697;&#32423;&#36733;&#20307;&#65292;&#25105;&#20204;&#20351;&#29992;&#23427;&#26469;&#26367;&#25442;PMG36e&#20013;&#30340;&#32418;&#38665;&#25239;&#24615;
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Much research has verified that tripeptides initiated with a branched-chain aliphatic amino acid residue and terminated with a proline have a strong antihypertensive activity in vivo. However, it is difficult to release from their precursor proteins that are orally administered. The dipeptides FR described here may be widely used as linkers to help the release of the active peptides with proline at C-terminus from their protein precursors by ACE or gastrointestinal enzymes in human body.
  
 
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<partinfo>BBa_K3142003 parameters</partinfo>
 
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==2019SZPT-IGEM团队==
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===Reference===
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我们构建了具有nisi抗性的质粒,下图是经过我们替换了EMR抗性后的质粒图。
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*Sheng-qi Rao, Song Liu, Tao Ju, et, al. Design of substrate-type ACE inhibitory pentapeptides with an antepenultimate C-terminal proline for efficient release of inhibitory activity.[J] Biochemical Engineering Journal. 60 (2012) 50– 55
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[[File:nisi gene.png|500px|center]]
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<center>'''nisi基因bp为801bp'''</center>
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==列表展示==
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<ul>
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<li>nihao</li>
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<li>wohao</li>
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<li>dajiahao</li>
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<li><a href="#">这是一个超链接</a></li>
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</ul>
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http://2017.igem.org/Team:Baltimore_Bio-Crew/Experiments
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<br/>
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([http://2012.igem.org/Team:Bielefeld-Germany/Protocols/Materials#Autoinduction_medium autoinduction] and manual induction)
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==nisi平板筛选==
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[[File:nisinshaixuan.png|500px|center]]
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<center>'''左边为带有nisin抗性的平板，右边为没有没有构建的菌'''</center>
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<div>左边是带有40IU/ML的nisin平板，我们将左边的菌做了菌落PCR，得出来的电泳图证明了我们构建成功。</div>
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Latest revision as of 16:43, 20 October 2019


FR

Much research has verified that tripeptides initiated with a branched-chain aliphatic amino acid residue and terminated with a proline have a strong antihypertensive activity in vivo. However, it is difficult to release from their precursor proteins that are orally administered. The dipeptides FR described here may be widely used as linkers to help the release of the active peptides with proline at C-terminus from their protein precursors by ACE or gastrointestinal enzymes in human body.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

  • Sheng-qi Rao, Song Liu, Tao Ju, et, al. Design of substrate-type ACE inhibitory pentapeptides with an antepenultimate C-terminal proline for efficient release of inhibitory activity.[J] Biochemical Engineering Journal. 60 (2012) 50– 55