Difference between revisions of "Part:BBa K2957023"
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What does it do?: It's responsible for the constitutive expression of the reporter, mKate2. It was important for our project in particular because it was able to show successful transfection in HEK cells | What does it do?: It's responsible for the constitutive expression of the reporter, mKate2. It was important for our project in particular because it was able to show successful transfection in HEK cells | ||
| − | How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. See more on the Parts Overview Page or MIT iGEM 2019 wiki: | + | How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. See more on the Parts Overview Page or MIT iGEM 2019 wiki: (https://2019.igem.org/Team:MIT/Parts) |
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Latest revision as of 16:38, 20 October 2019
hEF1a, mKate2
Contents: hEF1a, mKate2, SynthpA, with Carb resistance
What is it?: A Composite part; it is a genetic circuit with hEF1a promoter and mKate2 reporter allowing for identifiable expression.
What does it do?: It's responsible for the constitutive expression of the reporter, mKate2. It was important for our project in particular because it was able to show successful transfection in HEK cells
How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. See more on the Parts Overview Page or MIT iGEM 2019 wiki: (https://2019.igem.org/Team:MIT/Parts)
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 964
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 964
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 964
Illegal AgeI site found at 81
Illegal AgeI site found at 970 - 1000COMPATIBLE WITH RFC[1000]