Difference between revisions of "Part:BBa K3147004:Design"

 
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__NOTOC__
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<partinfo>BBa_K3147003 short</partinfo>
  
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<partinfo>BBa_K3147003 SequenceAndFeatures</partinfo>
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===Design note===
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The TEV cutting site used is the conventional site cleaved composed of 5 amino acids: ENLYFQ.
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We have observed that this construction is weakly expressed. We advise future users to add a RiboJ insulator and a BCD2 element to improve its expression.
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A double terminator controls the expression of this parts [[Part:BBa_B0015]].
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This sequence was synthesized by IDT DNA for iGEM Headquarters.

Latest revision as of 16:30, 20 October 2019

mRFP1 fused to a TEV-cleavable ssrA tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 691
  • 1000
    COMPATIBLE WITH RFC[1000]


Design note

The TEV cutting site used is the conventional site cleaved composed of 5 amino acids: ENLYFQ.

We have observed that this construction is weakly expressed. We advise future users to add a RiboJ insulator and a BCD2 element to improve its expression.

A double terminator controls the expression of this parts Part:BBa_B0015.

This sequence was synthesized by IDT DNA for iGEM Headquarters.