Difference between revisions of "Part:BBa K3286046"

 
 
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<partinfo>BBa_K3286046 short</partinfo>
 
<partinfo>BBa_K3286046 short</partinfo>
  
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Monomeric Red fluorescent protein and Green fluorescent protein downstream of bacteriophage lambda PL, PRM and PR promoter (including the operator regions OL and OR and the 5'UTR's).  
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Monomeric Red fluorescent protein (mRFP) [[part:BBa_K3286045]] and Green fluorescent protein (GFP) [[BBa_E0040]] downstream of bacteriophage lambda PL, PRM and PR promoter regions (including the operators OL and OR, and the 5'UTR's) [[part:BBa_K3286045]].  
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This part has been used to asses the potential of bacteriophage lambda inhibition by CRISPRi, by targeting the essential, early promoters.  
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This part has been used to asses the potential of bacteriophage lambda inhibition by CRISPRi, by targeting essential early promoters.  
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<ul><p>Atsumi, S., & Little, J. W. (2004). Regulatory circuit design and evolution using phage λ. Genes and Development, 18(17), 2086–2094. https://doi.org/10.1101/gad.1226004</p>
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<p>Court, D. L., Oppenheim, A. B., & Adhya, S. L. (2007). A new look at bacteriophage lambda genetic networks. Journal of Bacteriology, 189(2), 298–304. </p>
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<p>Liu, X., Jiang, H., Gu, Z., & Roberts, J. W. (2013). High-resolution view of bacteriophage lambda gene expression by ribosome profiling. Proceedings of the National Academy of Sciences, 110(29), 11928–11933. https://doi.org/10.1073/pnas.1309739110</p>
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<p>Ptashne, M. (2004). A genetic switch : phage lambda revisited. In Cold Spring Harbor Laboratory Press Cold Spring Harbor NY (Vol. 3).</p>
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<p>Wilson, H. R., Kameyama, L., Zhou, J. G., Guarneros, G., & Court, D. L. (1997). Translational repression by a transcriptional elongation factor. Genes and Development, 11(17), 2204–2213. https://doi.org/10.1101/gad.11.17.2204</p>
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</ul>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 16:06, 20 October 2019


mRFP-GFP divergent, Bacteriophage Lambda promoters, operators and 5'UTR's

Monomeric Red fluorescent protein (mRFP) part:BBa_K3286045 and Green fluorescent protein (GFP) BBa_E0040 downstream of bacteriophage lambda PL, PRM and PR promoter regions (including the operators OL and OR, and the 5'UTR's) part:BBa_K3286045. This part has been used to asses the potential of bacteriophage lambda inhibition by CRISPRi, by targeting essential early promoters.


    Atsumi, S., & Little, J. W. (2004). Regulatory circuit design and evolution using phage λ. Genes and Development, 18(17), 2086–2094. https://doi.org/10.1101/gad.1226004

    Court, D. L., Oppenheim, A. B., & Adhya, S. L. (2007). A new look at bacteriophage lambda genetic networks. Journal of Bacteriology, 189(2), 298–304.

    Liu, X., Jiang, H., Gu, Z., & Roberts, J. W. (2013). High-resolution view of bacteriophage lambda gene expression by ribosome profiling. Proceedings of the National Academy of Sciences, 110(29), 11928–11933. https://doi.org/10.1073/pnas.1309739110

    Ptashne, M. (2004). A genetic switch : phage lambda revisited. In Cold Spring Harbor Laboratory Press Cold Spring Harbor NY (Vol. 3).

    Wilson, H. R., Kameyama, L., Zhou, J. G., Guarneros, G., & Court, D. L. (1997). Translational repression by a transcriptional elongation factor. Genes and Development, 11(17), 2204–2213. https://doi.org/10.1101/gad.11.17.2204


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 7
    Illegal AgeI site found at 119
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1723