Difference between revisions of "Part:BBa K3286044"

 
 
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<partinfo>BBa_K3286044 short</partinfo>
 
<partinfo>BBa_K3286044 short</partinfo>
  
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Red fluorescent protein (RFP) and Green fluorescent protein (GFP) downstream of bacteriophage Lambda promoters, UTR's and operator regions OL and OR.
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The native bacteriophage Lambda promoters (PL, PRM and PR), operator regions (OL1,2,3 and OR1,2,3) and 5' UTR (with RNase III sensitive hairpin-loop and NutL site). The PL and PR promoter can be regulated by the CI and the CRO protein, which is a native lambda transcribed protein.  
  
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This construct contains the regulatory regions and promoters of bacteriophage Lambda and has been used to asses a for the potential inhibition of bacteriophage LAmbda by CRISPRi.  
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<ul><p>Atsumi, S., & Little, J. W. (2004). Regulatory circuit design and evolution using phage λ. Genes and Development, 18(17), 2086–2094. https://doi.org/10.1101/gad.1226004</p>
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<p>Court, D. L., Oppenheim, A. B., & Adhya, S. L. (2007). A new look at bacteriophage lambda genetic networks. Journal of Bacteriology, 189(2), 298–304. </p>
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<p>Liu, X., Jiang, H., Gu, Z., & Roberts, J. W. (2013). High-resolution view of bacteriophage lambda gene expression by ribosome profiling. Proceedings of the National Academy of Sciences, 110(29), 11928–11933. https://doi.org/10.1073/pnas.1309739110</p>
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<p>Ptashne, M. (2004). A genetic switch : phage lambda revisited. In Cold Spring Harbor Laboratory Press Cold Spring Harbor NY (Vol. 3).</p>
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<p>Wilson, H. R., Kameyama, L., Zhou, J. G., Guarneros, G., & Court, D. L. (1997). Translational repression by a transcriptional elongation factor. Genes and Development, 11(17), 2204–2213. https://doi.org/10.1101/gad.11.17.2204</p>
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</ul>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 16:01, 20 October 2019


Bacteriophage Lambda PL, PRM and PR promoters, operators and 5' UTR's

The native bacteriophage Lambda promoters (PL, PRM and PR), operator regions (OL1,2,3 and OR1,2,3) and 5' UTR (with RNase III sensitive hairpin-loop and NutL site). The PL and PR promoter can be regulated by the CI and the CRO protein, which is a native lambda transcribed protein.


    Atsumi, S., & Little, J. W. (2004). Regulatory circuit design and evolution using phage λ. Genes and Development, 18(17), 2086–2094. https://doi.org/10.1101/gad.1226004

    Court, D. L., Oppenheim, A. B., & Adhya, S. L. (2007). A new look at bacteriophage lambda genetic networks. Journal of Bacteriology, 189(2), 298–304.

    Liu, X., Jiang, H., Gu, Z., & Roberts, J. W. (2013). High-resolution view of bacteriophage lambda gene expression by ribosome profiling. Proceedings of the National Academy of Sciences, 110(29), 11928–11933. https://doi.org/10.1073/pnas.1309739110

    Ptashne, M. (2004). A genetic switch : phage lambda revisited. In Cold Spring Harbor Laboratory Press Cold Spring Harbor NY (Vol. 3).

    Wilson, H. R., Kameyama, L., Zhou, J. G., Guarneros, G., & Court, D. L. (1997). Translational repression by a transcriptional elongation factor. Genes and Development, 11(17), 2204–2213. https://doi.org/10.1101/gad.11.17.2204

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]