Difference between revisions of "Part:BBa K3286043"

 
 
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<partinfo>BBa_K3286043 short</partinfo>
 
<partinfo>BBa_K3286043 short</partinfo>
  
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Native bacteriophage Lambda PL promoter, upstream of the CI (Lambda repressor) protein.
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The native bacteriophage Lambda PR and PRM promoter of bacteriophage Lambda, with the operator regions OR1, OR2 and OR3, and the PR 5'UTR.  
  
 
The PRM promoter can be regulated by the native bacteriophage lambda proteins CI and CRO. The CI protein regulates the RPM promoter in a positive sense by creating a docking station for RNA polymerase and the CRO protein can regulated the PRM promoter in a negative sense by shielding the promoter region for binding of RNA polymerase.
 
The PRM promoter can be regulated by the native bacteriophage lambda proteins CI and CRO. The CI protein regulates the RPM promoter in a positive sense by creating a docking station for RNA polymerase and the CRO protein can regulated the PRM promoter in a negative sense by shielding the promoter region for binding of RNA polymerase.
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===References===
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<ul><p>Atsumi, S., & Little, J. W. (2004). Regulatory circuit design and evolution using phage λ. Genes and Development, 18(17), 2086–2094. https://doi.org/10.1101/gad.1226004</p>
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<p>Court, D. L., Oppenheim, A. B., & Adhya, S. L. (2007). A new look at bacteriophage lambda genetic networks. Journal of Bacteriology, 189(2), 298–304. </p>
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<p>Liu, X., Jiang, H., Gu, Z., & Roberts, J. W. (2013). High-resolution view of bacteriophage lambda gene expression by ribosome profiling. Proceedings of the National Academy of Sciences, 110(29), 11928–11933. https://doi.org/10.1073/pnas.1309739110</p>
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<p>Ptashne, M. (2004). A genetic switch : phage lambda revisited. In Cold Spring Harbor Laboratory Press Cold Spring Harbor NY (Vol. 3).</p></ul>
  
 
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Latest revision as of 15:59, 20 October 2019


Native bacteriophage Lambda PR and PRM promoter, OR operators and 5' UTR

The native bacteriophage Lambda PR and PRM promoter of bacteriophage Lambda, with the operator regions OR1, OR2 and OR3, and the PR 5'UTR.

The PRM promoter can be regulated by the native bacteriophage lambda proteins CI and CRO. The CI protein regulates the RPM promoter in a positive sense by creating a docking station for RNA polymerase and the CRO protein can regulated the PRM promoter in a negative sense by shielding the promoter region for binding of RNA polymerase.

References

    Atsumi, S., & Little, J. W. (2004). Regulatory circuit design and evolution using phage λ. Genes and Development, 18(17), 2086–2094. https://doi.org/10.1101/gad.1226004

    Court, D. L., Oppenheim, A. B., & Adhya, S. L. (2007). A new look at bacteriophage lambda genetic networks. Journal of Bacteriology, 189(2), 298–304.

    Liu, X., Jiang, H., Gu, Z., & Roberts, J. W. (2013). High-resolution view of bacteriophage lambda gene expression by ribosome profiling. Proceedings of the National Academy of Sciences, 110(29), 11928–11933. https://doi.org/10.1073/pnas.1309739110

    Ptashne, M. (2004). A genetic switch : phage lambda revisited. In Cold Spring Harbor Laboratory Press Cold Spring Harbor NY (Vol. 3).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]