Difference between revisions of "Part:BBa K2934008"
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This part is made to produce the enzyme Glucose Oxidase (GOx) by <em>Bacillus subtilis</em> in a regulated manner. The part contains the Lac operon inhibitor [https://parts.igem.org/wiki/index.php?title=Part:BBa_K143033 (BBa_K143033)], under the regulation of KatA promoter [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2934002 (BBa_K2934002)]. At growing concentrations of hydrogen peroxide, the suppression of KatA promoter is reduced, bringing to the production of LacI, which will suppress the production of GOx (under the regulation of pLac [https://parts.igem.org/wiki/index.php?title=Part:BBa_K143015 BBa_K143015]). The GOx enzyme production in this circuit has negative feedback.
 
This part is made to produce the enzyme Glucose Oxidase (GOx) by <em>Bacillus subtilis</em> in a regulated manner. The part contains the Lac operon inhibitor [https://parts.igem.org/wiki/index.php?title=Part:BBa_K143033 (BBa_K143033)], under the regulation of KatA promoter [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2934002 (BBa_K2934002)]. At growing concentrations of hydrogen peroxide, the suppression of KatA promoter is reduced, bringing to the production of LacI, which will suppress the production of GOx (under the regulation of pLac [https://parts.igem.org/wiki/index.php?title=Part:BBa_K143015 BBa_K143015]). The GOx enzyme production in this circuit has negative feedback.
  
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[[file:T--Technion-Israel--HoneyCircuitFeedback.png|400px|thumb|center|figure 1: our design of the Honey Circuit, made by iGEM Technion 2019 team BeeFree]]
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[[file:T--Technion-Israel--HoneyCircuitFeedback.png|500px|thumb|center|figure 1: our design of the Honey Circuit, made by iGEM Technion 2019 team BeeFree]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 15:01, 20 October 2019


pKatA-LacI-pLac-Glucose Oxidase-Histag for B. subtilis

Design

This part is made to produce the enzyme Glucose Oxidase (GOx) by Bacillus subtilis in a regulated manner. The part contains the Lac operon inhibitor (BBa_K143033), under the regulation of KatA promoter (BBa_K2934002). At growing concentrations of hydrogen peroxide, the suppression of KatA promoter is reduced, bringing to the production of LacI, which will suppress the production of GOx (under the regulation of pLac BBa_K143015). The GOx enzyme production in this circuit has negative feedback.

figure 1: our design of the Honey Circuit, made by iGEM Technion 2019 team BeeFree

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1268
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1564
    Illegal BsaI site found at 1895
    Illegal BsaI.rc site found at 1841
    Illegal BsaI.rc site found at 2666