Difference between revisions of "Part:BBa K2984063"
(→Usage and Biology) |
|||
| (6 intermediate revisions by 3 users not shown) | |||
| Line 3: | Line 3: | ||
<partinfo>BBa_K2984063 short</partinfo> | <partinfo>BBa_K2984063 short</partinfo> | ||
| − | Level | + | Level 2 backbone |
| + | |||
| − | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | <html> | ||
| + | |||
| + | This part is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. The backbone is desgined for MoClo level 2 cloning. Together with the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984003">L1a</a>, <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984005">L1b</a> and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984002"> L1c </a> three different level 1 constructs can be inserted into the level 2 backbone. The backbone contains an antibiotic selection-cassette so this backbone can directly be used for transformations into <i>C. reinhardtii</i>. This unit consists of the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008"> Psad </a> promoter, the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984006"><i>aphVIII</i> resistance gene for paromomycin resistance</a> and the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984018"> Rbcs2 </a> terminator. It is flanked by an N-terminal <i>BamHI</i> and a C-terminal <i>XhoI</i> restriction site to allow the exchange of the selection-cassette. Herefore our <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984056"> hygromycin construct </a> or <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984037"> ptxD construct </a> | ||
| + | could be inserted instead. The selection-cassette benfits the transformation rate of Chlamydomonas since it makes co-transformations obsolete. It is not necessary to clone all three level 1 construct into the backbone. It could be reduced to one or two using one of our <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984062"> Level 2 Linker </a> </html> | ||
| − | |||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K2984063 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2984063 SequenceAndFeatures</partinfo> | ||
Latest revision as of 14:57, 20 October 2019
Phytobrick Level 2 backbone (with C.reinhardtii paromomycin marker)
Level 2 backbone
Usage and Biology
This part is a part of the Chlamy-HUB-Collection. The backbone is desgined for MoClo level 2 cloning. Together with the L1a, L1b and L1c three different level 1 constructs can be inserted into the level 2 backbone. The backbone contains an antibiotic selection-cassette so this backbone can directly be used for transformations into C. reinhardtii. This unit consists of the Psad promoter, the aphVIII resistance gene for paromomycin resistance and the Rbcs2 terminator. It is flanked by an N-terminal BamHI and a C-terminal XhoI restriction site to allow the exchange of the selection-cassette. Herefore our hygromycin construct or ptxD construct could be inserted instead. The selection-cassette benfits the transformation rate of Chlamydomonas since it makes co-transformations obsolete. It is not necessary to clone all three level 1 construct into the backbone. It could be reduced to one or two using one of our Level 2 Linker
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BamHI site found at 1121
Illegal XhoI site found at 3012 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal AgeI site found at 189
Illegal AgeI site found at 301 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.