Difference between revisions of "Part:BBa K2970003:Design"

 
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
Green, A.A. et al. Complex cellular logic computation using ribocomputing devices. Nature 548, 117-121 (2017) doi:10.1038
+
Green, A.A. et al. "Complex cellular logic computation using ribocomputing devices." (Nature, <b>2017</b>)
  
 
===References===
 
===References===

Revision as of 14:21, 20 October 2019


Trigger 1 Composition


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The triggers were produced using primers and then inserted with golden gate assembly into the backbone with a strong promoter and terminator. As backbone pSB1C3 was used, from which first BsaI sites were removed and then BsmBI sites were added to cut out the ampicillin resistance later.


Source

Green, A.A. et al. "Complex cellular logic computation using ribocomputing devices." (Nature, 2017)

References