Latest revision as of 13:33, 20 October 2019

pGAP-Kozak-FLO10 pro-pelA-His tag-TT-Pgpda-Kozak-FLO10-SLAC-His tag-TT-Pgas-Alkali-TT

We combine pelA, SLAC genes, which each adds His tag behind together.Through the previous experiment, we find the signal peptide which is most suitable for the target genes, and form the final pathway.Besides we add an alkali peptide to maintain pH.

Figure1 path way

Characterization

This is a three section for degrade banana fiber part.

Usage and Biology

We put pectinase pelA at the first place because the banana fiber is covered by pectin. After dealing with the surface of the fiber, SLAC is as follow to degrade lignin inside the banana further, aiming to gain higher quality of the fiber. Since all these three enzymes’ optimum pH is near 5, we designed a pH system. We use Pgas, a promoter activate at pH equals 5, matching the optimum pH of the enzymes. In case the whole system’s pH decreases too much, we use Pgpda, a promoter activate at pH equals 2, connecting it with an alkali peptide to stabilize pH.

Sequence and Features