Difference between revisions of "Part:BBa K3196034"
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Pgpda is a promoter amplified from the genome of Aspergillus niger, which can achieve maximum response at around pH5.0 and express the corresponding coding sequence. We can test the function of the promoter by measuring the fluorescence intensity at different pH gradients and get the related parameters. After testing the promoter, replace the GFP protein with the target gene. | Pgpda is a promoter amplified from the genome of Aspergillus niger, which can achieve maximum response at around pH5.0 and express the corresponding coding sequence. We can test the function of the promoter by measuring the fluorescence intensity at different pH gradients and get the related parameters. After testing the promoter, replace the GFP protein with the target gene. | ||
| − | + | <h1>'''Usage and Biology'''</h1> | |
| + | We cultured Pichia pastoris containing PgpdA-gfp in pH=4/5/6 medium for 4 hours, and then the fluorescence of each tube was observed under UV lamp. The results showed that it had better expression effect under the condition of pH=5: | ||
| + | [[File:T--HUST-China--2019-Pgpda |400px|thumb|center|Figure1.pH5 shows higher fluorescence intensity ]] | ||
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Revision as of 13:31, 20 October 2019
Pgpda-GFP
Pgpda is a promoter amplified from the genome of Aspergillus niger, which can achieve maximum response at around pH5.0 and express the corresponding coding sequence. We can test the function of the promoter by measuring the fluorescence intensity at different pH gradients and get the related parameters. After testing the promoter, replace the GFP protein with the target gene.
Usage and Biology
We cultured Pichia pastoris containing PgpdA-gfp in pH=4/5/6 medium for 4 hours, and then the fluorescence of each tube was observed under UV lamp. The results showed that it had better expression effect under the condition of pH=5:
File:T--HUST-China--2019-Pgpda
Figure1.pH5 shows higher fluorescence intensity
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 207
Illegal BamHI site found at 850 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]