Difference between revisions of "Part:BBa K3081031"
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<partinfo>BBa_K3081031 short</partinfo>
 
<partinfo>BBa_K3081031 short</partinfo>
  
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This part is an improvement of <partinfo>BBa_K3081008</partinfo>, which we add a degradation signal peptide ssrA to the dCas9. This  largely accelerates the degradation rate of dCas9 and weakens its overinhibtion on genome replication initiation targeted to the R1 box.
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This part is an improvement of <partinfo>BBa_K3081008</partinfo>, which we add a degradation signal peptide “ssrA” to the dCas9. This  largely accelerates the degradation rate of dCas9 and weakens its overinhibtion on genome replication initiation targeted to the R1 box.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 13:26, 20 October 2019


pBAD-dCas9-ssrA-J23119-R1+

This part is an improvement of BBa_K3081008, which we add a degradation signal peptide “ssrA” to the dCas9. This largely accelerates the degradation rate of dCas9 and weakens its overinhibtion on genome replication initiation targeted to the R1 box.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 2321
    Illegal NheI site found at 5495
    Illegal NheI site found at 5518
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 4600
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961