Difference between revisions of "Part:BBa K2922043:Design"
 
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DsbA comes from Escherichia coli (Strain K12) and PbBD is a putative Pb2+ binding domain derived from PbrR.
 
DsbA comes from Escherichia coli (Strain K12) and PbBD is a putative Pb2+ binding domain derived from PbrR.
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===Reference===
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1. C. Hui et al., Surface display of PbrR on Escherichia coli and evaluation of the bioavailability of lead associated with engineered cells in mice. Sci Rep 8, 5685 (2018).
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<br>
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2. C. Y. Hui, Y. Guo, X. Q. Yang, W. Zhang, X. Q. Huang, Surface display of metal binding domain derived from PbrR on Escherichia coli specifically increases lead(II) adsorption. Biotechnol Lett 40, 837-845 (2018).

Latest revision as of 13:00, 20 October 2019


Expression system of DsbA-PbBD coding periplasm display of lead binding peptide with T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 328
    Illegal NotI site found at 1472
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1385
    Illegal BamHI site found at 521
    Illegal XhoI site found at 883
    Illegal XhoI site found at 1132
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 663
    Illegal NgoMIV site found at 1588
    Illegal NgoMIV site found at 2906
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 567


Design notes

We ordered the part DNA from Sangon ® company.


Source

DsbA comes from Escherichia coli (Strain K12) and PbBD is a putative Pb2+ binding domain derived from PbrR.


Reference

1. C. Hui et al., Surface display of PbrR on Escherichia coli and evaluation of the bioavailability of lead associated with engineered cells in mice. Sci Rep 8, 5685 (2018).
2. C. Y. Hui, Y. Guo, X. Q. Yang, W. Zhang, X. Q. Huang, Surface display of metal binding domain derived from PbrR on Escherichia coli specifically increases lead(II) adsorption. Biotechnol Lett 40, 837-845 (2018).