Difference between revisions of "Part:BBa K2970009"

Revision as of 12:16, 20 October 2019

J23100 Test Composition with RiboJ

Since the ribosome binding site can influence the promoter strength, we thought about an option to separate it from the promoter. For this we used the ribozyme RiboJ, which always makes the same cut between the promoter and the following sequences. Thus this plasmid can be used to measure promoters. In this case the promoter J23100 was inserted into the backbone No part name specified with partinfo tag. for characterization.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 789

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2970009 short</partinfo>
-Since the RBS can influence the promoter strength, it may be necessary to separate it from the promoter. For this we used RiboJ, which always makes the same cut in front of the promoter. Thus this plasmid can be used to measure promoters. In this case the promoter J23100 was inserted for measurement.
+Since the ribosome binding site can influence the promoter strength, we thought about an option to separate it from the promoter. For this we used the ribozyme RiboJ, which always makes the same cut between the promoter and the following sequences. Thus this plasmid can be used to measure promoters. In this case the promoter J23100 was inserted into the backbone <partinfo>pSB1C3-BBa§0240</partinfo> for characterization.
 <!-- Add more about the biology of this part here